thiazole orange staining

thiazole orange staining,

Thiazole Orange | Sigma-Aldrich

Sigma-Aldrich offers a number of Thiazole Orange products. View information & documentation regarding Thiazole Orange, including CAS, MSDS & more.

Section 8.1 - Nucleic Acid Stains - mobitec

The three classes of classic nucleic acid stains (Table 8.4) include: • Intercalating dyes, such as ethidium bromide and propidium iodide • Minor-groove binders, such as DAPI and the Hoechst dyes • Miscellaneous nucleic acid stains, including acridine orange, 7-AAD, LDS 751 and hydroxystilbamidine, with special properties

thiazole orange staining,

Thiazole orange | CAS 107091-89-4 | SCBT - Santa Cruz .

Buy Thiazole orange (CAS 107091-89-4), a fluorescent dye for DNA staining, from Santa Cruz. Molecular Formula: C26H24N2O3S2, Molecular Weight: 476.61

Rapid Enumeration of Live and Dead Bacteria by . - DESATOYA

BD Biosciences Immunocytometry Systems 2350 Qume Drive, San Jose, CA 95131 .bdbiosciences Rapid Enumeration of Live and Dead Bacteria by Flow Cytometry Using Thiazole Orange, Propidium Iodide and Counting Beads William Godfrey, Rana Alsharif, Martin Tapia, and Diether Recktenwald

Demonstration That Thiazole-Orange-Positive . - Blood

Demonstration That Thiazole-Orange-Positive Platelets in the Dog Are Less Than 24 Hours Old By George L. Dale, Paul Friese, Laurie A. Hynes, and A. Burstein Approximately 6% of dog platelets are positive for staining with thiazole orange, a dye frequently used to stain ribo- nucleic acid.

SYBR Safe - Wikipedia

SYBR Safe has a very similar structure to thiazole orange, which has a methyl group attached to the charged nitrogen, whereas SYBR Safe has an N-propyl group. Thiazole Orange has been shown to be three to four times less mutagenic than ethidium bromide whereas SYBR Safe …

Thiazole orange *CAS 107091-89-4* | AAT Bioquest

Thiazole orange is a fluorescent DNA-binding dye for reticulocyte analysis. It is lso useful for Plasmodium species analysis. The modified thiazole orange derivatives (such as SYBR Green, PicoGreen, TOTO and YOYO) are widely used for detecting DNA samples.

Thiazole Orange as an Alternative to Antibody Binding for .

often exhibit distinctive staining patterns along metaphase chromosomes dependent upon features, including binding to the major and minor DNA grooves, level of chromatin compaction, nucleotide specificity, and level of dye stacking. Relatively recently, the fluorochrome Thiazole Orange (TO) was shown to preferentially bind to triplex DNA in gels.

Evaluation of the interference of platelets in .

The conducted studies indicated the presence of platelet interference with the method of reticulocyte enumeration by flow cytometry using thiazole orange for cell staining. This interference can be excluded by a combination of specific antibody and the establishment of a gate in the scatter plot analysis, improving the accuracy of the obtained .

orange 2 | Sigma-Aldrich

1.46425 ; Application: Bacterial count and confirmation of the presence of acid-tolerant microorganisms in food packaging materials and beverages Packaging: 200 ml in 250 ml bottle with blue screw cap and 3 loci (6 bottles per box) Millipore

Fluorophore - Wikipedia

A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds.. Fluorophores are sometimes used alone, as a tracer in fluids, as a dye for staining of certain structures, as a substrate of enzymes, or .

SYBR Safe - Wikipedia

SYBR Safe has a very similar structure to thiazole orange, which has a methyl group attached to the charged nitrogen, whereas SYBR Safe has an N-propyl group. Thiazole Orange has been shown to be three to four times less mutagenic than ethidium bromide whereas SYBR Safe …

BD Cell Viability Kit - bdbiosciences

cytometry. The kit contains thiazole orange (TO) solution to stain all cells and propidium iodide (PI) to stain dead cells. BD Liquid Counting Beads is a liquid suspension of fluorescent beads. Add the beads to a flow sample to calculate absolute counts. The kit can be ordered with or without counting beads. The method provides a

Improved specificity of determination of immature .

Improved specificity of determination of immature erythrocytes (reticulocytes) by multiparameter flow-cytometry and thiazole orange using combined staining with monoclonal antibody (anti-glycophorin-A). Serke S(1), Huhn D.

Thiazole Orange Homodimer, 1 mM in DMSO | Biotium

Thiazole orange homodimer (TOhD), also know as TOTO®-1, is a cell-impermeant, high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer. The dye is essentially nonfluorescent in the absence of nucleic acids, but shows bright green fluorescence when bound to nucleic acids. λ Ex /λ Em (with DNA): 520/514 nm; Orange solution

FLUORESCENCE MICROSCOPY ANALYSIS OF . - Purdue University

Protocol for Acridine Orange Staining after DNA denaturation . Acridine Orange (AO) is a metachromatic dye which differentially stains double-stranded (ds) and single-stranded (ss) nucleic acids. When AO intercalates into dsDNA it emits green fluorescence upon excitation at 480-490 nm. On the contrary, it emits red when interacts with ssDNA or RNA.

thiazole orange staining,

DNA Electrophoresis Using Thiazole Orange Instead of .

Thiazole orange compares favorably to common staining methods, in that it is sensitive, inexpensive, excitable with UV or blue light (to prevent sample damage), and safer than ethidium bromide. Labs already equipped to run DNA electrophoresis experiments using ethidium bromide can generally switch dyes with no additional changes to existing .

A molecular fluorescent dye for specific staining and .

A new RNA-selective fluorescent dye integrated with a thiazole orange and a p-(methylthio)styryl moiety shows better nucleolus RNA staining and imaging performance in live cells than the commercial stains. It also exhibits excellent photostability, cell tolerance, and counterstain compatibility with 4′,6-dia

Comparison of Methodologies for Detecting Reticulated .

analyzer using polymethyl oxazine staining, and a flow cytometer using thiazole orange staining. The correlation between the two methods was analyzed. The advantages and disadvantages of the two methods were clarified. Statistical analysis for the reference ranges in the two methods was conducted.

[Cytometry] Thiazole Orange/Hydroethidine Plasmodium/RBC

Delynn Moss wrote- > We have limited experience in staining P. falciparum in human RBCs with thiazole orange (TO) and hydroethidine (HE). Our issue is that TO and HE give different autofluorescence from uninfected RBCs that have been exposed to TO only and HE only.

Thiazole - Wikipedia

Thiazole, or 1,3-thiazole, is a heterocyclic compound that contains both sulfur and nitrogen; the term 'thiazole' also refers to a large family of derivatives. Thiazole itself is a pale yellow liquid with a pyridine-like odor and the molecular formula C 3 H 3 NS.

Molecular Engineering of Thiazole Orange Dye: Change of .

The universal fluorescent staining property of thiazole orange (TO) dye was adapted in order to be specific for G-quadruplex DNA structures, through the introduction of a styrene-like substituent at the ortho-position of the TO scaffold. This extraordinary outcome was determined from experimental studies and further explored through molecular docking studies.

METHODOLOGY Open Access Cytometric measurement of …

Methods: The method is based on the staining of parasites with hydroethidine (HE) and thiazole orange (TO) which allow differential identification of early, trophozoite and late stage of the parasite by flow cytometry. Late stages of the parasites are obtained by incubation in culture for 24 hours. Reinvasion is followed by culturing

Halogen-containing thiazole orange . - Beilstein-Institut

2902 Halogen-containing thiazole orange analogues – new fluorogenic DNA stains Aleksey€A.€Vasilev*1, Meglena€I.€Kandinska2, Stanimir€S.€Stoyanov2, Stanislava€B.€Yordanova2, David€Sucunza3, Juan€J.€Vaquero3, Obis€D.€Castaño3, Stanislav€Baluschev4 and€Silvia€E.€Angelova*3,5 Full Research Paper Open Access Address:

Thiazole orange: A new dye for reticulocyte analysis - Wiley

Staining and Analysis A stock solution of thiazole orange (1 mg/mL) was made in methanol. The stock solution was diluted 1:10,000 in phosphate-buffered saline containing 0.002 M EDTA and 0.02% sodium azide. The diluted dye solu- tion was stable for at least 1 wk when stored in the dark at room temperature.

A New Approach to Detect Reticulated . - ScienceDirect

Mar 15, 2000· Lee et al. stained reticulocytes with Thiazole Orange (T.O.) and demonstrated that cell membranes were highly permeable to T.O., and that T.O. has a high affinity for both RNA and DNA. Their results indicated that T.O. was an adequate dye for staining reticulated platelets that contained an abundance of RNA under certain conditions.

Double staining of Plasmodium falciparum . - DeepDyve

Jan 01, 2004· Read "Double staining of Plasmodium falciparum nucleic acids with hydroethidine and thiazole orange for cell cycle stage analysis by flow cytometry, Cytometry" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at …

Thiazole orange: A new dye for reticulocyte analysis - Lee .

The best results were obtained with a dye we have named "thiazole orange"; analysis of several blood samples with thiazole orange gave a correlation coefficient of 0.97 as compared to a manual determination of reticulocyte percentage.

Nucleic Acid (DNA, RNA) Quantification and RNA/DNA Ratio .

Staining with Hoechst clearly gave much lower DNA yields. As a consequence, RNA concentrations would be overestimated in this case. As we do not know the contribution of the damaged DNA to the fluorescence detected with thiazole orange, thiazole orange fluorescence cannot be used for correction of the DNA and RNA estimates.